目的 探讨经颅磁电刺激(TMES)疗法对海仁酸(KA)致颞叶癫痫鼠的疗效。 方法 62只大鼠按照随机数字表法分为预处理组(共32只)和治疗组(共30只)，预处理组再分为4个小组，分别给予治疗仪最大电流强度(MCI) 0%，25%，50%，75%的电流刺激，确定本实验条件下的最佳刺激参数。治疗组再分为3个小组，每组10只。2组(癫痫刺激组、癫痫非刺激组)为符合纳入标准的癫痫模型大鼠，刺激组刺激参数为预处理组确定出的最佳刺激参数；非刺激组大鼠刺激前后处理与刺激组相同，但治疗仪无有效的能量输出。第3组对照组为未造模对照大鼠。3组大鼠均给予每天1次，40 min/次，共14 d的刺激。记录并比较3组大鼠行为学、电生理学及组织学变化，以评估TMES疗法对癫痫大鼠的疗效。 结果 50%MCI为最佳刺激强度。癫痫刺激组大鼠癫痫波出现的频率明显低于癫痫非刺激组大鼠[(30.210 ±4.580)次/min vs. (31.380 ± 4.247)次/min]，差异有统计学意义(t=3.235，P=0.001)。Timm染色结果提示3组大鼠染色评分差异有统计学意义(F=17.429, P=0.000)，癫痫刺激组大鼠海马齿状回的内分子层Timm染色评分较癫痫非刺激组明显减轻，差异有统计学意义(P＜0.05)。结论 TMES疗法可通过改善癫痫大鼠海马齿状回内分子层组织学变化程度来影响齿状回神经元环路的形成，进而降低癫痫脑电波的发作频率。

Alzheimer’s disease (AD) has been reported for more than 100 years since its first discovery in 1906. There has been no significant progress in the study of its real causes and pathogenesis. The viewpoint of this paper is a heuristic viewpoint based on brain cell activation theory under such background. In this paper, the pathogenesis of sporadic AD is discussed at molecular level by applying the principles of cell physics and biology. The purpose of this paper is to harmonize the existing theories of etiology of AD and to solve the source problems that have plagued the research field of neurodegenerative diseases for a long time. Conclusion: (1) Basic contents: excess cations are transferred from extracellular to intracellular. They compete position with potassium ions on the inner surface of cell membranes, thus abatementing the membrane potential, making action potential unable to activate calcium channels normally, which eventually leads to abnormal apoptosis of brain cells. Amyloid plaque is the remains of abnormal apoptotic brain cells. Amyloid plaque is the aggregation of amyloid spots by van der Waals force and electrostatic attraction, and its interstitium is amyloid protein. Brain cells consist of neurons, microglia and astrocytes in turn. Most of the spotted nuclei in the remains are cations. (2) Solutions: physical means, especially transcranial magnetoelectricity stimulation, should be the first choice for treatment. (3) Naming principles: because of the core point of view in this paper—The position of cation occupying potassium is the initiating factor of AD, so the etiology theory is named theory of dove-like particles. Take the Chinese idiom “Turtledove occupies the nest of magpies”. “Turtledove” represents the non-essential cation, “magpie” refers to the effective potassium ion, and “nest” refers to the position of the effective potassium ion.

This is a new idea that based on effective treatment of Parkinson's disease and Alzheimer's disease with transcranial magnetoelectric stimulation technology, it can understand a hypothesis about voltage-gated Ca2+ channels is the best target for activation by physical means, basic content：Parkinson's disease , Alzheimer's disease etc. neuronal degeneration diseases, that closely related to physical-gated ion channels, which can be treated with physical means, activating neurotransmitters-energic neurons plays key roles in the treatment, and voltage-gated Ca2+ channels is the best target for physical means, the purpose is to induce Ca2+ inflowing and triggers neuronal axon terminals synaptic vesicles releasing neurotransmitters. The theory of brain cell activation sets forth the principle, method and purpose of treatment of the physical gated ion channel diseases such as Alzheimer's disease, Parkinson's disease and other neural degeneration diseases, and indicates that the attempt to treat these diseases using pharmaceutical and chemical approaches could shake our confidence in conquering the diseases, and the application of physical approaches or combined application of physical and chemical approaches in the treatment of some major encephalopathy may be our main research direction in the future.

In order to reasonably explain the phenomenon of cell bioelectricity, we proposed the conservation law of cell membrane area, established the ion inequality equation, and therefore paid attention to the mystery of “θ-τ”. We researched and analyzed the “θ-τ” mystery, discussed the parity non-conservation in weak interactions, suggested possible experiments to test the parity non-conservation in weak interactions, and gave our research and analysis conclusions: The parity non-conservation in weak interactions, is still a “conjecture”; The experimental scheme suggested in the papers by C. N. Yang et al. cannot determine whether the weak interaction can separate left and right, and it is impossible to directly answer whether θ and τ in the “θ-τ” mystery are the same particle; The Co60 β decay experiment such as C. S. Wu is a pseudo-mirror experiment, whether the experimental result violates parity conservation is only based on the assumption of C. N. Yang et al. In fact, experiments such as polarized Co60 did not overturn the so-called “law of parity conservation”. The mirror image principle does not have any physical meaning, does not correspond to any physical conservation quantity, and cannot be destroyed by any physical experiment. In the process of turning “mirror symmetry” and “mirror asymmetry” into so-called physical “common sense” and scientific “facts” respectively, the methods of transformation are “stealing concepts” and “circular argumentation”. The “θ-τ” mystery is a “man-made” mystery. θ and τ are two different particles, which may be the result of the same precursor particle being divided into two. The work of C. N. Yang, T. D. Lee, C. S. Wu et al. has brought quantum physicists from the “small black room” to the “bigger black room” or “smaller black room”. The right and wise choice is to go back through the door that came in. With the development of science today, it is time for some contents to reform from the bottom.

本文提出的钾离子通道模型，是一个独立的功能单位，四条孔道螺旋同步单方向旋转，转运K+方式是被动单向的，对ATP没有依赖性，不同于之前的“船桨”“螺旋桨”“旋转门”等模型。作用机制是K+与孔道螺旋内带正电荷的氨基酸形成的排斥力推动“风齿”后退“风车”旋转，而K+通道孔径大小随着“风车”转速的快慢而变化，决定着通道孔“开放”与“关闭”。“折纸风车”模型，是应用细胞的生物物理学原理揭示K+通道运行机制，对与之相关的其它一些基础性研究具有启发意义，或许有助于从源头上回答人类健康和疾病的基本生物学问题。