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期刊论文
Culture of Escherichia coli in SOC medium improves the cloning efficiencyof toxic protein genes
Analytical Biochemistry 394(2009)144-146，-0001，（）：
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which isa glucose-containing rich medium, significantly improved the transformation efficiency of a recombinantplasmid containing a toxic plant subtilase SaSBT1 cDNA. Both glucose and rich nutrients present in the SOC medium prevented the unintended activation of the lac promoter carried on the cloning vector, and led to significantly improved transformation efficiency of recombinant plasmids containing toxicprotein genes and an increased rate of transformant growth.
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