Knots and links have been conjectured to play a fundamental role in a wide range of scientific fields. Recently, tying isolated vortex knots in the complex optical field has been realized. However, how to construct the acoustic vortex knot is still an unknown problem. Here we propose theoretically and demonstrate experimentally the creation of acoustic vortex knots using metamaterials, with decoupled modulation of transmitted phase and amplitude. Based on the numerical simulation, we find that the knot function can be embedded into the acoustic field by designed metamaterials with only 24x24 pixels. Furthermore, using the optimized metamaterials, the acoustic fields with Hopf link and trefoil knot vortex lines have been observed experimentally. Although knots in complex optical fields have been realized experimentally, the realization of acoustic vortex knots is still problematic. Here, the authors have demonstrated the creation of acoustic vortex knots by embedding the knot function into a propagating acoustic field using a metasurface hologram.