Protective Role of D-Lactate Dehydrogenase in Doxorubicin-Induced Cardiomyocyte Injury

• 1、Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China

Abstract：Aim: Investigation of the protective role of D-lactate dehydrogenase (Ldhd) in doxorubicin (DOX)-induced cardiomyocyte injury. Methods: Cardiomyocyte injury model was established by DOX treatment in AC16 and H9C2 cells and the Ldhd levels were measured before and after the injury. Further, the Ldhd expression was upregulated and downregulated in AC16 via plasmid transient transfection and siRNA interference, respectively, and the effects on the oxidative stress and mitochondrial function were examinedwith live-cell immunofluorescence staining. Results: (1) The Ldhd expression was significantly upregulated by the DOX-induced injury. (2) The DOX treatment resulted in the elevation of ROS production and declination of mitochondrial membrane potential. The overexpression of Ldhd attenuated both the tendencies, while the knockdown worsened both. Conclusions: These findings suggest that Ldhd may contribute to the protection of injured cardiomyocytes, potentially through the suppression of oxidative stress and preservation of mitochondrial function. Further investigation is warranted to explore its relevance as a therapeutic target in cardiac diseases.

Keywords： D-lactate dehydrogenase doxorubicin cardiomyocyte injury oxidative stress mitochondrial function