Angiopoietin-2-mediated Interactions between Von Willebrand Factor and 95D Cells

• 1、Cyrus Tang Hematology Center,Soochow University,Suzhou,215123

Abstract：Objective: To verify the mediation role of Angiopoietin-2 (Ang-2) in the interaction between von Willebrand Factor (VWF) and highly metastatic human lung cancer cells (95D). Methods: Flow cytometry was employed to assess the expression levels of Ang-2 and Tie-2 on the surface of 95D cells,as well as the binding capacity of VWF and its various domains with 95D cells. Cell adhesion assays were further conducted to measure the binding between VWF and 95D cells. Enzyme-linked immunosorbent assay (ELISA) was utilized to detect the interaction between Ang-2 and the VWF A1 domain. The binding sites between VWF, its A1 domain, and 95D cells were blocked using antibodies against Ang-2 and inhibitors of Tie-2. Results: Expression of Ang-2 and Tie-2 was detected on the surface of 95D cells. VWF could directly interact with 95D cells, primarily through the VWF A1 domain. ELISA results showed that Ang-2 binds to the VWF A1 domain with high affinity. Blocking the binding sites of VWF and Ang-2 with Ang-2 antibodies and Tie-2 inhibitors significantly weakened the binding between VWF and 95D cells. Conclusion: Ang-2 mediates the interaction between VWF and 95D cells, providing a new therapeutic target for cancer treatment and drug research.

Keywords： VWF Ang-2 95D