Combined Treatment with Astragalus Polysaccharide, Clostridium Butyricum ,and Metformin for Liver Injury Induced by Simulated Weightlessness in Mice with Humanized Intestinal Flora

• 1、School of Bioengineering, Jiangnan University, Wuxi 214122, China
• 2、Research Department, The Ninth Medical Center of Chinese PLA General Hospital, Beijing 100101, China
• 3、The Affiliated Hospital of Jiangnan University, Wuxi 214000, China

Abstract：Objective To investigate the therapeutic effects of a combination of Atragalus polysaccharide, Clostridium butyricum, and metformin on liver injury induced by simulated weightlessness over a 30-day period in mice with humanized intestinal flora. Methods A total of sixty-four male C57BL/6J mice, aged 10 weeks, were randomly assigned and evenly distributed into eight distinct groups.umanized intestinal flora mice were developed to replicate the human intestinal environment. These mice were suspended by their tails for 30 days to simulate a weightless condition. During this period, they received gavage treatment with a mixture of Astragali polysaccharides, Clostridium butyricum, and metformin.t the conclusion of the experiment, fecal samples were collected from the mice for the analysis of intestinal flora, while blood samples were obtained to assess biochemical levels of blood lipids and liver function. Results Compared to the humanized blank group, the serum levels of AST, ALT, and IL-6 in the humanized hanging tail group were significantly elevated (P < 0.05). Conversely, the levels of HDL-C and TP were markedly reduced (P < 0.05).Serum levels of AST and IL-6 were significantly reduced (P < 0.05) in the humanized suspension tail drug group when compared to the humanized suspension tail group.Simulated weightlessness resulted in a decrease in the abundance of Dubosiella and an increase in the content of Bacteroides. Following drug treatment, both the abundance of Dubosiella and Bacteroides were found to be reduced.Simulated weightlessness significantly increased the abundance of GCA-900066575 while markedly decreasing the abundance of Desulfovibrio and A2. Additionally, drug treatment led to a significant reduction in the abundance of Ligilactobacillus and Dubosiella, whereas the abundances of Bacteroides, Lactobacillus, and Helicobacter were significantly elevated.Conclusion The combined treatment of Astragali polysaccharide, Clostridium butyricum, and metformin demonstrated significant reparative effects on liver injury induced by simulated weightlessness conditions in mice.

Keywords： Simulated weightlessness Humanized mice Intestinal flora Liver injury repair