基于细胞吸附与离子交换色谱的高纯度Nisin Z回收新策略

董浩; 张建华; 张世杰; 陈旭升; 王靓; 张宏建

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Novel Approach for High-Purity Nisin Z Recovery Utilizing Cell-Bound Adsorption and Ion-Exchange Chromatography

首发时间：2025-03-13

Dong Hao ^{1
2
3
4}
Dong Hao（1999, male, main research direction: fermentation engineering）
Zhang Jianhua ^{1
2
3
4}
Zhang Jianhua（1969), Male, Professor, master's supervisor,mainly engaged in research and clean production technology
Zhang Shijie ^{1
2
3
4} Chen Xusheng ^{1
2
3
4} Wang Liang ^{1
2
3
4} Zhang Hongjian ^{1
2
3
4}

1、Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122 Jiangsu
2、 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu
3、 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu
4、School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu

Abstract：Nisin is a natural antimicrobial peptide widely used in the food industry owing to its effectiveness and safety. High-purity nisin has shown significant potential for medical applications, including cancer treatment, skin infections, and oral health, owing to its enhanced bioactivity and safety. However, conventional purification methods often suffer from low efficiency, which makes it challenging to achieve high purity and recovery simultaneously. This study introduced an innovative purification process specifically designed to produce high-purity nisin. This process involved nisin adsorption onto microbial cells during fermentation, with approximately 48% of the nisin bound to the cells. After fermentation, 0.02 M hydrochloric acid was used to desorb cell-bound nisin, purified via weak acid cation-exchange chromatography with D155 resin. A novel salt-free hydrochloric acid-ethanol solution was employed as the eluent, achieving over 95% elution efficiency and yielding nisin with a specific activity of 80,216.43 international units (IU)/mg protein. The freeze-dried product obtained from the ion-exchange chromatography elution represented high-purity nisin Z, with a 36,372.66 IU/mg titer. Stability tests over three batch runs confirmed the reproducibility of the process, which achieved a high-purity nisin yield of 94.42%. This streamlined process substantially enhanced the purity and yield of nisin, offering a scalable approach for industrial production in the pharmaceutical field.

keywords： separation and purification high-purity nisin ion-exchange chromatography cell-bound nisin desorption

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基于细胞吸附与离子交换色谱的高纯度Nisin Z回收新策略

董浩 ^{1
2}
Dong Hao（1999, male, main research direction: fermentation engineering）
张建华 ^{1
2}
Zhang Jianhua（1969), Male, Professor, master's supervisor,mainly engaged in research and clean production technology
张世杰 ^{1
2} 陈旭升 ^{1
2} 王靓 ^{1
2} 张宏建 ^{1
2}

1、江南大学工业生物技术教育部重点实验室江苏无锡 214122
2、江南大学生物工程学院江苏无锡 214122

摘要：Nisin是一种天然抗菌肽，因其有效性和安全性广泛应用于食品工业。高纯度Nisin因其增强的生物活性和安全性，在医学领域展现出显著潜力，包括癌症治疗、皮肤感染和口腔健康。然而，传统的纯化方法效率较低，导致难以在保证高纯度的同时实现高回收率。本研究提出了一种创新的纯化工艺，专门用于生产高纯度nisin。该工艺包括在发酵过程中通过微生物细胞吸附nisin，其中约48% 的nisin和细胞结合。发酵后，使用0.02 M盐酸解吸细胞结合的Nisin，并通过D155树脂进行弱酸阳离子交换层析纯化。采用了一种新型无盐盐酸-乙醇溶液作为洗脱剂，实现了超过95%的洗脱效率，并获得了比活性为80,216.43国际单位(IU)/mg蛋白的nisin。通过离子交换层析洗脱得到的冷冻干燥产品代表了高纯度的nisin Z，比活性为36,372.66 IU/mg。三批次稳定性测试确认了该工艺的可重复性，获得了94.42%的高纯度nisin产率。该简化工艺显著提高了nisin的纯度和产率，为制药领域工业化生产提供了可扩展的解决方案。

关键词：分离纯化高纯度nisin 离子交换层析菌体结合nisin

基金：

1. This study was supported by the Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China （KLIB-KF202206 and KLIB-KF202204)）

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Dong Hao,Zhang Jianhua,Zhang Shijie,et al. Novel Approach for High-Purity Nisin Z Recovery Utilizing Cell-Bound Adsorption and Ion-Exchange Chromatography[EB/OL]. Beijing:Sciencepaper Online[2025-03-13]. https://www.paper.edu.cn/releasepaper/content/202503-107.

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论文编号	202503-107
论文题目	基于细胞吸附与离子交换色谱的高纯度Nisin Z回收新策略
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