Establishment of a zebrafish hemolytic anemia model and preliminary exploration of the efficacy of polysaccharides from Lycium barbarum

• 1、School of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan Province 410114

Abstract：This article established a zebrafish hemolytic anemia model and explored the efficacy of polysaccharides from Lycium barbarum (LBP) using this model. The hemolytic anemia model was established by incubating zebrafish with phenylhydrazine. The suitable conditions were discovered based on the results of survival rate and cardiac red blood cell staining. To further investigate this model, the survival rate, morphological development, motor behavior, and tissue healing ability were analyzed. Finally, the improvement effect of LBP on hemolytic anemia in zebrafish was preliminarily explored. The results showed that the suitable conditions for establishing the zebrafish hemolytic anemia model were treating 2dpf (days post fertilization) zebrafish with 2 μmol/L phenylhydrazine for 24 hours. Compared with the control group, the survival rate and morphological development of zebrafish were not significantly changed in the hemolytic anemia group. However, the abilities of spontaneous movement and tissue healing were significantly inhibited by hemolytic anemia. After treated with LBP, the number of cardiac red blood cell of zebrafish with hemolytic anemia showed a significant increase. In summary, this study established a zebrafish hemolytic anemia model and confirmed the improvement effect of LBP on hemolytic anemia, providing new experimental basis and research ideas for studies on hemolytic anemia and efficacy exploration of active factors such as LBP.

Keywords： hemolytic anemia zebrafish spontaneous movement tissue healing phenylhydrazine LBP