The role and mechanism of MafF in the repair of skeletal muscle ischemia reperfusion injury

• 1、Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, P. R. China

Abstract：Objective: The aim of this study is to investigate the role and its underlying mechanism of MafF in muscle regeneration. Methods: By using wild-type satellite cells and constructing ischemia-reperfusion injury model, the localization of MafF cells and the change of its expression after IR injury were investigated. The effect of MafF on proliferation and differentiation of C2C12 myoblasts was investigated by constructing a MafF overexpressing C2C12 myoblast line. Through ChIP and dual luciferase reporting experiments, the regulatory relationship between MafF and myogenic related factors was investigated. Results: MafF is expressed in satellite nucleus and is up-regulated immediately after IR injury, suggesting that MafF is involved in the repair process of IR injury in skeletal muscle. Overexpression of MafF did not affect the proliferation of C2C12 cells, and ChIP experiments showed that MafF could not target the expression of PAX7, suggesting that MafF\'s involvement in muscle injury and regeneration is not associated with the proliferation of satellite cells. MyoD, a determinant of early differentiation of satellite cells, can bind to the MafF promoter. After overexpression of MafF, it was found that MafF was positively correlated with the late differentiation factor Myogenin, and could target and activate the expression of Myogenin.

Keywords： Cell Biology Satellite cells MafF Ischemia reperfusion injury MyoD Myogenin