Prokaryotic expression, purification and characterization of laccase from the fungus Cyathus bulleri

• 1、Changchun University of Science and Technology,College of Life Sciences and Technology Changchun, Jilin

Abstract：Laccase is a copper-containing polyphenol oxidase, belonging to the blue polycopper oxidase family, capable of oxidizing, a wide range of aromatic compounds and other organic compounds and reducing oxygen to form water. Laccase catalyzes a wide range of substrates, and thus has great potential for applications in paper bleaching, bioremediation, dye decolorization, clarification of fruit juices and wines, and textile cleaning. Laccase is prone to form inclusion bodies when heterologously expressed in Escherichia coli, and soluble protein expression is very low. In this paper, the fusion of laccase with an anchoring peptide (GDGDD) and co-expression with the molecular chaperonin MA4386 were used to improve the soluble expression of the target protein in E. coli, and finally a high-purity laccase with biological activity was obtained.

Keywords： Laccase Prokaryotic expression Molecular chaperonin