The effects of aGVHD on the morphology and structure of murine intestinal blood vessels and its underlying mechanism

• 1、Cyrus Tang Hematology Research Center, Soochow University

Abstract：To explore the relationship and mechanism between the changes of intestinal tissue blood vessels and intestinal immune homeostasis in mice during the course of aGVHD. We established a mouse aGVHD model, and analyzed the dynamic changes of endothelial cells, pericyte and infiltrating immune cells in mouse small intestinal villi at different time points. qPCR and Smart-seq were used to search for differentially expressed genes. It was found that in the course of aGVHD, the damage of small intestinal villus vessels and the changes of infiltrating immune cells were closely related to the course of the disease, and the expression of inflammatory response, apoptosis and intestinal barrier related genes were significantly changed. According to the results of the study, we believe that the severe damage and structural disorder of villi vessels induced by aGVHD may be an important cause of receptor death, and the aGVHD inflammatory response of villi may damage the integrity of villi vessels and improve their permeability mainly by affecting gene expression related to endothelial cell apoptosis and intestinal barrier.

Keywords： Immunity1 Acute graft-versus-host disease2 Inflammatory response3 Intestinal vessels4 Endothelial cells5 Pericytes6