枸杞提取物与植物乳杆菌M5的共微胶囊化及体外评价
首发时间:2023-04-06
摘要:该文将枸杞提枸杞提取物与植物乳杆菌M5的共微胶囊化及体外评价取物与植物乳杆菌M5共微胶囊化制备高活性益生菌微胶囊并对其进行体外评价。结果表明:高活性植物乳杆菌微胶囊中枸杞提取物最佳浓度为3 g/100mL,枸杞-植物乳杆菌微胶囊在4±1 ℃和25±1 ℃条件下储藏4周后,细胞活力分别提高12.14%、18.27%,枸杞提取物可促进植物乳杆菌的生长,表现出良好的益生元效果,且枸杞提取物的添加不影响微胶囊中细胞在模拟胃肠消化过程中的保留和释放。体外肠道培养24 h后,添加枸杞-植物乳杆菌微胶囊显著增加肠道菌群丰富度和多样性,总菌属、拟杆菌属和双歧杆菌属数量均明显增加(P<0.05),分别增加7.74%、6.43%和15.01%,同时肠道中短链脂肪酸乙酸、丙酸、丁酸和异戊酸含量分别增加36.55%、25.54%、24.05%和41.81%,表明枸杞-植物乳杆菌微胶囊可有效增加肠道中有益菌数量,产生有益的代谢产物,从而维持肠道微生态平衡。因此,枸杞提取物与植物乳杆菌共微胶囊化在新型功能性益生菌食品开发方面具有巨大潜力。
关键词: 植物乳杆菌 枸杞提取物 微胶囊 储藏 胃肠消化 肠道菌群
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Co-microencapsulation Goji berry extract and Lactobacillus plantarum M5 and in vitro evaluation
Abstract:Highly active probiotic microcapsules were prepared and evaluated in vitro by co-microencapsulating Goji berry extract with Lactobacillus plantarum M5. The results showed that the optimum concentration of Goji berry extract in the highly active Lactobacillus plantarum microcapsule was 3 g/100mL. After 4 weeks of storage at 4±1 ℃ and 25±1 ℃, the cell viability of Goji berry- Lactobacillus plantarum microcapsules increased by 12.14% and 18.27%, respectively. Goji berry extract could promote the growth of Lactobacillus plantarum, exhibiting good prebiotic effects, and the addition of Goji berry extract did not affect the retention and release of cells in the microcapsule during simulated gastrointestinal digestion. After 24 hours of intestinal culture in vitro, theCo-microencapsulation Goji berry extract and Lactobacillus plantarum M5 and in vitro evaluation abundance and diversity of intestinal flora were significantly increased (P<0.05) with the addition of Goji berry-Lactobacillus plantarum microcapsules, the number of total bacteria, Bacteroides, and Bifidobacterium were increased by 7.74%, 6.43%, and 15.01%, respectively. At the same time, the contents of short-chain fatty acid acetic acid, propionic acid, butyric acid and isovaleric acid in the intestine were significantly increased (P<0.05) by 36.55%, 25.54%, 24.05% and 41.81% respectively. This indicated that Goji berry-Lactobacillus plantarum microcapsules could effectively increase the number of beneficial bacteria in the intestinal tract, produce beneficial metabolites, and maintain the intestinal microecological balance. In conclusion, the co-microencapsulation Goji berry extract and Lactobacillus plantarum has great potential in the development of new functional probiotic foods.
Keywords: Lactobacillus plantarum Goji berry extract microencapsulation storage gastrointestinal digestion gut microbiota
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