Application of loop-mediated isothermal amplification technology combined with electrochemical detection in the detection of food-borne pathogens

• 1、College of Biology,Hunan University,Changsha 410082

Abstract：Food-borne pathogens exist in the living environment, and there are great hidden dangers to the safety of food. Common food-borne pathogens The pathogenic bacteria are Escherichia coli, Staphylococcus aureus, Salmonella, etc. If young children and elderly people with low immunity are infected, it will cause related serious symptoms. Therefore, the timely detection of food-borne pathogens plays a vital role in controlling food safety and preventing diseases from entering the mouth. Compared with polymerase chain reaction , loop-mediated isothermal amplification technology does not rely on sophisticated instrument support. While ensuring high sensitivity, rapidity and specificity, it can achieve the purpose of visualization and multiple detection, which is convenien to the testing work in poverty-stricken areas. This paper mainly introduces the amplification principle of loop-mediated isothermal amplification technology and its application in electrochemical detection of five common food-borne pathogens.

Keywords： foodborne pathogens loop-mediated isothermal amplification electrochemical detection