Effects of Inoculation and Culture Conditions on the Growth and Reproduction of PWN

• 1、School of Biotechnology，Jiangsu University of Science and Technology，Zhenjiang 212100
• 2、The Sericulture Research Institute, Chinese Academy of Agriculture Sciences, Zhenjiang 212008
• 3、Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute，Chinese Academy of Agricultural Sciences, Zhenjiang 212008

Abstract：The culture effect of pine wood nematode (Bursaphelenchus xylophilus,PWN) was investigated from the concentration of culture medium, inoculation quantity, preservation time and inoculation method. The results showed that in the early stage of inoculation, the number of nematodes isolated from the medium was less than the inoculation amount, except for the treatment of direct inoculation. PWN can also grow on low concentration medium, but the number of reproduction is small. The time of nematode breeding in the medium was longer when the inoculation quantity was less. In the treatment with large inoculation amount, the nematodes propagated rapidly and the experimental time became shorter, and a large number of nematodes crawled to the cover of the culture dish which caused the counting error and the collection of nematodes was inconvenient. In each preservation period, fresh (just cultured) PWN had the best vitality. When the nematodes were directly inoculated into the new culture medium from the original culture medium, the population of the nematodes was fast and large. The reproduction speed and quantity of wood nematode on the medium are related to many factors, such as medium concentration, inoculation amount, storage time and storage method, but they are not strictly one-to-one correspondence.

Keywords： Forest Protection Bursaphelenchus xylophilus Method of inoculation Culture conditions