Extraction of water-soluble and fat-soluble components with three liquid phase system

• 1、Department of Pharmaceutical Engineering, College of Chemistry & Chemical Engineering, Chongqing University, Chongqing, China, 401331

Abstract：Water-soluble lithospermic acid B (LAB) and fat-soluble tanshinone IIA (Tan IIA) are main bioactive components in Salvia miltiorrhiza Bunge which has anti-atherosclerosis, anti-neuritis, anti-tumor and other pharmacological effects. Traditional industrial methods for extracting LAB and Tan IIA have the problems of high costs, low efficiency and cumbersome operation. In this paper, n-hexane-ethanol-NaH2PO4-H2O three liquid phase system (TLPS) was used to extract LAB and Tan IIA simultaneously. TLPS extraction was optimized to achieve optimal condition: aqueous NaH2PO4 solution (2.50mol/L, pH 2.00): ethanol: n-hexane=38:38:24 (v/v/v), temperature 47.0?C and time 12.00 h. The total yields of LAB and Tan IIA were 146±9.8 mg/g and 0.538±0.027 mg/g, which were 128% and 52.8% higher than the single phase methods in the pharmacopoeia, respectively. The thermodynamics and kinetics in the extraction and separation of LAB and Tan IIA were analyzed and discussed.

Keywords： Three liquid phase system simultaneous extraction lithospermic acid B tanshinone IIA