4-α-糖基转移酶在淀粉基热可逆凝胶制备中的应用
首发时间:2020-01-06
摘要:淀粉凝胶安全无毒、易于消化的优点使其广泛应用于食品加工的各个行业中。目前淀粉凝胶由于其凝胶不可逆,无法循环利用,也限制了其应用。因此,为了得到淀粉基热可逆凝胶,本研究筛选不同的淀粉作为底物,利用作者所在实验室前期构建的Thermoproteus uzoniensis来源的4-α-糖基转移酶(4αGT)对其进行改性。结果表明,最适反应底物为马铃薯淀粉,马铃薯淀粉在75oC出现了明显的热可逆现象。反应过程中直链淀粉含量逐渐降低。经改性后,马铃薯淀粉的冻融稳定性较原淀粉相比显著提高,经五次冻融循环后析水率由34.2%降低至10%以下。链长分布结果表明,A链和B1链的含量降低明显,B2链和B3链含量有着不同程度的升高。
关键词: 4-α-糖基转移酶 淀粉改性 热可逆凝胶 冻融稳定性
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Application of 4-α-glucotransferase in the preparation of starch-based thermo-reversible gel
Abstract:The safety, nontoxicity and easy digestion of starch made it widely used in food processing. In recent years, the unthermo-reversibility limit the application of starch gel. In order to obtain a starch-based thermoreversible gel, six different starches were chosen as the substrates and modified with 4-α-glucotransferase (4αGT) derived from Thermoproteus uzoniensis. The results showed that the optimal reaction substrate was potato starch. An obvious thermo-reversibility was observed at 75ºC in modified potato starch.After modification, the freeze-thaw stability of potato starch was significantly improved compared to the original starch. After five freeze-thaw cycles, the water precipitation rate reduced from 34.2% to less than 10%. The chain length distribution results showed that the contents of A and B1 chains significantly decreased, and the contents of B2 and B3 chains increased to varying degrees.
Keywords: 4-α-glucotransferase Starch modification Thermo-reversible gel Freeze-thaw stability
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4-α-糖基转移酶在淀粉基热可逆凝胶制备中的应用
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