不同来源的副干酪乳杆菌的基因组特征及其抗生素耐受性研究
首发时间:2019-02-11
摘要:以分离自不同地区人群粪便样品和泡菜样品的33株副干酪乳杆菌为研究对象,对其基因组进行特征分析,包括泛基因组和核心基因组概况、系统进化关系以及抗生素抗性基因,同时对所有菌株进行了12种抗生素的耐受性测定。基因组分析结果显示,副干酪乳杆菌的泛基因组是开放的,核心基因组较为集中;分离源的地域因素对副干酪乳杆菌的遗传进化有较高的影响;不同副干酪乳杆菌携带抗生素抗性基因的数目没有显著差异。抗生素耐受性实验结果显示,部分分离自人粪便样品的菌株对克林霉素、阿莫西林、环丙沙星、红霉素和四环素均存在抗性,而分离自泡菜样品的菌株普遍对抗生素较为敏感;菌株FCQNA42L2和FCQNA38L1对克林霉素、FCQHC12L3对四环素均表现出最高抗性,可能与这些菌株携带克林霉素抗性基因ErmB和四环素抗性基因tetM有关。
关键词: 副干酪乳杆菌 泛基因组 核心基因组 系统进化 抗生素耐受
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Genomic characteristics and antibiotic resistance of Lactobacillus paracasei isolated from different sources
Abstract:In this study the genomes of 33 strains of Lactobacillus paracasei (L. paracasei) were characterized by pan-genome and core genome analysis, phylogenetic relationships and antibiotic resistance genes. The tolerance to 12 antibiotics was also performed for all the 33 strains. The results showed that the pan-genome of L. paracasei was open and the core genome was concentrated. The isolation source had significant effects on the genetic evolution of L. paracasei. There were no significant differences in the number of genes related to antibiotic resistance among the strains. Some strains isolated from human feces were resistant to clindamycin, amoxicillin, ciprofloxacin, erythromycin and tetracycline, while the strains isolated from pickles were generally more sensitive to the antibiotics. The strains FCQNA42L2, FCQNA38L1 and FCQHC12L3 showed strong resistance to clindamycin, clindamycin and tetracyclin, respectively, which might be related to the existence of genes Ermb for clindamycin and tetM for tetracyclin.
Keywords: Lactobacillus paracasei pan-genome core genome phylogeny antibiotic tolerance
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不同来源的副干酪乳杆菌的基因组特征及其抗生素耐受性研究
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