Cloning and Characterization of OfMYB1 and OfMYB2 genes from Osmanthus fragrance

• 1、Key Laboratory for Biology of Horticultural Plants，Ministry of Education，Huazhong Agricultural University，Wuhan 430070，China

Abstract： Osmanthus fragrance, belonging to the Oleaceae family, is a well-known ornamental and economically important, aromatic woody plant with a long history and is one of the ten most famous traditional plant in China. With the expressed sequence tags from transcriptome assembly and RACE method, two MYB transcription factors , which may involving in the biosynthesis of aroma and flower pigmentation, were obtained from petals of Osmanthus fragrans. The ORF of these two MYB genes were separately 588bp and 915bp, encoded 195 and 304 amino acid and named OfMYB1 and OfMYB2. Sequence and phylogenetic analysis showed that, OfMYB1 has an obvious R2R3-MYB domain, a highest homology with snapdragon AmMYB305, and a conserved motif nyWsV/ME/DDlWP/S in C-terminal. OfMYB2 has a R1-MYB domain and a highest homology with StMYB1R-1 from potato (Solanum tuberosum), belonging to the CCA1-likeⅡ subfamily. Experiment showed that the two MYB gene were located in nucleus. The results of time and space expression patterns by real-time PCR showed that OfMYB1 had the highest amount of expression in the petals, while no expression in young leaves, comparing to peduncles, pistils and stamens. Besides, it's showed a significant circadian rhythm, reaching the peak expression at 12:00. OfMYB2 were expressed in different expression levels in different varieties of flowering stages and in different tissues, also having a certain circadian variation.

Keywords： ornamental plant and horticulture Osmanthus fragrance OfMYB1 OfMYB2 gene clone and expression ananlysis