拟南芥小GTP结合蛋白RABE1C参与植物干旱胁迫调控研究
首发时间:2016-07-19
摘要:干旱是限制植物生长发育的主要逆境因子,严重影响作物产量从而造成重大经济损失。发掘和研究植物自身抗旱功能基因,是获取耐逆作物新品种和减轻逆境胁迫危害的重要途径,对于发展高效现代化农业有非常重要的推动作用。本研究发现,拟南芥小GTP结合蛋白RABE1C参与了植物干旱胁迫响应调控,其缺失表达突变体rabe1c-1及rabe1c-2对脱落酸(Abasic Acid, ABA)调控气孔运动过程不敏感,抗干旱能力降低。通过GUS染色分析,发现RABE1C在气孔中表达量较高;另外RABE1C受到外界干旱胁迫和ABA的诱导表达。结果综合分析表明,RABE1C可能作为一个正调控因子,通过调控气孔运动过程来参与植物干旱胁迫响应,进而影响植物的抗干旱能力。
关键词: 细胞生物学 干旱胁迫 脱落酸 气孔运动 RABE1C
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The Arabidopsis small GTP binding protein RABE1C positively regulates the plant drought tolerance
Abstract:Drought is one of the most serious stresses in restraining plant growth and development, as well as crop yield. To screen and investigate the plant drought responsive genes, is an important way to obtain the new essential functional genes and molecular mechanisms to improve plant drought tolerance and further development of water-use-efficiency agriculture. In this study, we found that Arabidopsis small GTP binding protein RABE1C is involved in plant drought response. Compared with wild type plants, the RABE1C loss-of-function mutants reduced the plant drought tolerance and showed hyposensitivity to ABA regulated stomatal movements. GUS staining assay showed that, RABE1C was highly expressed in a serilal of tissues, especially in guard cells. Moreover, RABE1C transcription level was induced by drought and ABA treatments. All presented data demonstrated that RABE1C may function as a positive regulator in ABA regulated stomatal movements, and thus to enhance the plant drought tolerance.
Keywords: Cell biology Drought stress Abscisic acid Stomatal movements RABE1C
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拟南芥小GTP结合蛋白RABE1C参与植物干旱胁迫调控研究
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