生物素-链霉亲合素系统介导的纳米材料标记和检测技术在生物分子检测中的应用
首发时间:2016-05-20
摘要:生物素-链霉亲和素系统(Biotin-(strept)avidin system, BAS)由于其高亲和性、高稳定性、高灵敏性、强特异性和极佳的生物相容性已广泛用于各种生物检测技术。BAS的应用可显著提高纳米材料的表面反应活性、生物相容性和分散稳定性。当用于细菌检测、病毒检测、细胞的分离、蛋白质检测以及核酸检测时,BAS介导的纳米材料不仅可有效改善目标分子对应配体的生物活性,使之与目标分子的结合效率明显提高,而且可将检测信号级联放大,有效提高检测灵敏度,缩短检测时间,实现快速、灵敏、超痕量生物分子检测,为生物检测和医学研究带来新的思路。
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Recent advances of biotin-streptavidin system mediated nanomaterials labelling and detection technology for detection of biological molecules
Abstract:Due to the high affinity, stability, sensitivity, specify and excellent biocompatibility makes biotin-(strept)avidin system widely applied in various of biological samples detection. BAS can improve the reaction activity of the nanoparticles surfaces, biocompatibility and dispersion stability of the nanoparticles. When BAS applied in cell separation, bacteria, virus, proteins and nucleic acid detection, it can improve the reaction activity of ligand and acceptor and lead to high binding efficiency. Most importantly, BAS mediated detection signal cascade amplification can improve the sensitivity and shorten the detection time. It can detect ultratrace biological molecules sensitivity and quickly which bring a new idea for biological detection and medical research.
Keywords: biological detection biotin streptavidin nanomaterials
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生物素-链霉亲合素系统介导的纳米材料标记和检测技术在生物分子检测中的应用
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