The affection of the level of Tgf-β2 gene by the influence the Clock gene in NIH3T3

• 1、Health Ministry Key Laboratory of Chronobiology, College of Basic Medicine and Forensic Medicine, Sichuan University,Chengdu 610041

Abstract：As one of the essential systems in a wide range of life forms, circadian system make life adapt to the external environmental and regulation of body homeostasis. Recently reporting that circadian genes which key of circadian regulation have some important affection in many other systems. It has been reported that the Clock gene plays an important role in the regulation of reproductive and development. To further explore the specific role and the mechanisms of the object, the study to NIH-3T3 as experimental subject, using RNA interference technology to reduce the Clock gene expression level in cells, and then through the PCR and Western-blot confirmed the Tgf-β2 which play an important role in variety of physical activities was significantly down-regulated. The result of Western-blot which to detect the levels of Clock and Tgf-β2 protein has confirmed this conclusion. The study confirmed the Clock gene expression in NIH-3T3 would affect the expression level of Tgf-β2 gene. The positive regulatory role in achieving this effect may have a further impact on the Tgf-β2-related physical activity and signaling pathways.

Keywords： Circadian rhythm Clock gene；Tgf-β2 gene；development