Analysis on two-component signal transduction system of the Pseudomonas fragi

• 1、College of Life Science,Nanjing Normal University,Nanjing, 210023

Abstract：Pseudomonas fragi was isolated from the extremly environment of Arctic with cold tolerance and salt tolerance. The two-component signal transduction system of Pseudomonas fragi was predicted and analyzed based on the whole genome sequencing. 13 pairs of TCSs and 2 orphan histidine protein kinases (HK) were identified, and the majority of HKs was found to have the HAMP, HATPase_c, HisKA, and other structural domains. Most of TCSs belong to OmpR sub family, accounted for more than 61.5% of all TCSs. The clustering analysis showed that The HisKAs located in the different evolutionary branch, suggesting the TCSs are diverse. The functions of TCSs in Pseudomonas fragi involved in nitrogen fixation and metabolism, heavy metals, copper resistance response regulation, and so on. This may contribute to the strain to survive in the Arctic environment. These results will lay the theoretical foundation for the cold tolerance and salt tolerance of Pseudomonas fragi in extreme conditions.

Keywords： Pseudomonas fragi Two-component signal transduction system Histidine protein kinase Phylogenetic analysis