Inducement and Culture of Transgenic Hair Roots and Functional Analysis of IFS Gene in Licorice

• 1、Agricultural University of Hebei/ North China Key Laboratory for Crop Germplasm Resources of Ministry of Education, Hebei, Baoding 071001, China

Abstract：Hair root system is widely used for gene function identification in molecular biology research. In this paper, the genetic transformation and cultural system of Licorice (Glycyrrhiza uralensis Fisch）hair roots integrated with IFS over-expressed vector was found as follows：take the cotyledonary nodes（3-4 days after germination）as the explants, co-cultured on the MS medium supplemented with 100 mg/L (AS) in darkness for 3-4d followed by being infected 30min with Aqua1 strain of Agrobacterium rhizogenes (OD600=0.5-0.7). Then transform to B5 medium supplemented with 300 mg/L Cef under light conditions to induce hairy root initiation. The transformation rate can reach to 34% according to green fluorescence result. HPLC analysis showed that the content of two isoflavones enhanced 1.7 and 1.9 times respectively in the hair root of IFS over-expressors comparing with the control. qPCR analysis proved that IFS in hairy roots was high efficiently expressed. The results would lay the foundation for revealing the molecular mechanism of glycyrrhizin accumulation and for further exploring the function of IFS gene.

Keywords： Licorice Transgenic hair roots Isoflavanone synthase