Different concentrations of GM-CSF effects on dendritic cells

• 1、Department of Hepatobiliary and pancreatic surgery ,The Second Affiliated Hospital of Kunming Medical University Yunnan,kunming650101,china

Abstract：Objective: To investigate the effects and the influences of granulocyte-macrophage colony stimulating factor with interleukin 4 on mouse bone marrow-derived dendritic like cells morphology, purity, number of affected cells, dendritic cells to explore the culture of the most suitable conditions. Methods: Dissociate and purification of mouse bone marrow mononuclear cell samples were added to different concentrations of GM-CSF and IL-4, its morphology and phenotype testing, as well as the ability to activate allogeneic lymphocytes measured. Results In a certain concentration range, with the increase of GM-CSF and IL-4 concentration, the number of mouse bone marrow-derived dendritic cells, the purity and maturity will also increase. The high concentrations of GM-CSF and IL-4 but prone to cause cell death. Accordingly, presumably ideal cells induce GM-CSF and IL-4 concentration range should be 10 ~ 20ng/ml and 2 ~ 50ng/ml. Conclusion: The optimum concentration of GM-CSF IL-4 can be combined to enhance the ability to induce murine bone marrow mononuclear cells into dendritic cell differentiation, cell stimulating rational use of these two factors, increase the number of dendritic cells, the purity and maturity.

Keywords： Dendritic cells culture in vitro Granulocyte - macrophage colony-stimulating factor Interleukin -4。