基于宏基因组分析四川泡菜母水作引子的泡菜发酵过程中细菌多样性变化
首发时间:2015-02-15
摘要:。四川泡菜是一种优良的传统发酵食品。研究以泡菜母水作引子的蔬菜发酵过程中细菌多样性变化,对于控制泡菜质量和泡菜中功能性细菌资源开发具有重要意义。此研究采集发酵过程中不同发酵时间的样品,提取基因组,PCR扩增16SrDNA的V3+V4区并进行Illumina高通量测序,分析结果比较发酵不同时间细菌多样性,结果表明:整个发酵过程中乳杆菌属起主导作用,总体上趋势为乳杆菌属所占比例先下降后上升至一定范围内保持稳定;发酵初期占比最高的是魏斯氏菌属,推测其对发酵启动具有重要作用。
关键词: 食品科学与工程 宏基因组 泡菜 发酵过程 细菌多样性
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Metagenomic analysis of bacterial diversity changes during vegetables fermentation using Sichuan pickled vegetables water as starter
Abstract:Sichuan pickled vegetables are good traditional fermented food. Studying the bacterial diversity during vegetables fermentation using Sichuan pickled vegetables water as starter is necessary for quality control of pickled vegetables and functional bacterial development. This study collected samples during the fermentation, extracted the genome of every sample, amplified the V3+V4 part of 16s rDNA by PCR and then sequenced using Illumina high throughout sequencing. Comparing the analysis of bacterial diversity of 6 samples, the results came out that Lactobacillus took most advantage during the fermentation, and showed first falling and after rising to a stable level. Weissella presented most at the beginning of the fermentation, and was inferred to play important role to star the fermentation.
Keywords: Food Science and Technology Metagenome pickled vegetables fermentation process bacterial diversity
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基于宏基因组分析四川泡菜母水作引子的泡菜发酵过程中细菌多样性变化
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