The Bioinformatics Analysis of Putative Tricarboxylic Acid Cycle-Related Proteins in Magnaporthe oryzae

• 1、The functional genomics center, Fujian Agriculture and Forestry University, Fujian Fuzhou, 350002

Abstract：Tricarboxylic acid cycle is hub material and energy metabolism and plays an important function in the life activities of aerobic organisms. However, the TCA cycle-related proteins in the plant pathogenic fungi, Magnaporthe oryzae, have not yet been identified and studied for their functions. In this study, we identified 18 putative TCA cycle-related proteins in M.oryzae by using 17 Saccharomyces cerevisiae proteins through BLAST search. The corresponding homologous protein contains the same functional domains in S. cerevisiae and M.oryzae,, the key proteins of TCA cycle in M. oryzae have the closest evolutionary relationship with Fusarium graminearum, Neurospora crassa and Aspergillus nidulans.by phylogenetic analysis. Based on the above results the TCA cycle metabolic network is drawn providing reference for further system analysis of rice blast fungus TCA cycle. In addition, all genes in TCA cycle expressed between appressorium and hyphal different stages, but there is obvious difference among genes.

Keywords： Magnaporthe oryza, Tricarboxylic acid cycle, Bioinformatics, Citrate synthase, Isocitrate dehydrogenase, α-ketoglutarate dehydrogenase