Anti-thrombogenic property of PCL/Chitosan hybrid small-diameter

• 1、Key Laboratory of Bioactive Materials,College of Life Science, Nankai University, Tianjin,300071

Abstract：Nowadays, vascular transplantation by using small-diameter vascular grafts is an effective strategy for the treatment of cardiovascular diseases. In clinic, no commercial product has been successfully utilized, because the thrombus formation and the subsequent occlusion. In this study, we developed a hybrid small-diameter vascular graft from synthetic polymer poly(ε-caprolactone) (PCL) and natural polymer chitosan by co-electrospinning technique (Dinner= 2mm). Heparin was immobilized onto the grafts through ionic bonding between heparin and chitosan fibers, thus introducing anti-thrombogenic function. The immobilization was relatively stable and heparin could continuously release from the grafts for more than one month. Heparin functionalization evidently improved the haemocompatibility of the PCL/CS vascular grafts, which was illustrated by the reduced platelet adhesion and prolonged coagulation time (APTT, PT, and TT) as shown in human plasma assay.

Keywords： functional polymer Small-diameter vascular grafts Heparin Controlled release Anti-thrombogenicity