Expression profile of global genome in offspring conceived through assisted reproductive technology

• 1、women's hospital, medical school, Zhejiang university

Abstract：Objective：To investigate the expression profile of whole genome in ART-conceived offspring and pick the key genes differentially expressed for further study. Method: Microarray analysis included 8 babies conceived through ART and 8 controls. Gene expression profiling was completed using Affymetrix U133 Plus 2.0 oligonucleotide microarrays. Significance analysis of microarray (SAM) was applied to detect the genes differentially expressed between the two groups. Hierarchical clustering, GO analysis and Pathway analysis was used to identify the characteristics of the differentially expressed genes. Gene expression data were validated by quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR). Forty ART babies and 40 controls were analyzed using real-time RT-PCR analysis. Result(s): Comparison of the gene profiles between ART babies and controls identified 159 differentially expressed genes. Hierarchical clustering showed that the gene profiles of ART babies and naturally conceived babies are distinct. GO analysis revealed that some genes involved in growth and development were down regulated in ART offspring. Pathway analysis highlighted several pathways including the toll like receptor signal pathway. To validate the gene expression data, eight genes were analyzed by real-time RT-PCR, which found that six gene were differentially expressed. Conclusion(s): This study provided the general expression profile of whole genome in ART-conceived offspring. These data provide a useful basis for understanding the molecular events leading to the increased risks in ART-conceived offspring and could be considered potential targets for further study.?????

Keywords： microarray real-time PCR clustering GO analysis pathway analysis