灰毛豆种子中化学成分的鉴定及其对黑翅土白蚁的杀虫活性

李冠华; 王苏宁; 丁文兵; 薛正杰; 刘自力; 李有志

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灰毛豆种子中化学成分的鉴定及其对黑翅土白蚁的杀虫活性

首发时间：2013-03-01

李冠华 ¹
李冠华，女，硕士，农艺师，生物源农药。
王苏宁 ¹ 丁文兵 ^{1
2} 薛正杰 ³ 刘自力 ³ 李有志 ^{1
2}
李有志，男，博士，副教授

1、湖南农业大学生物安全科技学院植物病虫害生物学与防控湖南省重点实验室，长沙 410128
2、国家植物功能成分工程技术研究中心，长沙 410128
3、长沙市白蚁防治站，长沙 410005

摘要：为探明灰毛豆Tephrosia purpurea种子中的杀虫成分及其对黑翅土白蚁Odontotermes formosanus工蚁成虫的杀虫活性，对其甲醇提取物采用多种色谱技术分离纯化，结合核磁共振图谱和质谱方法鉴定化合物的结构，并测定了各化合物对黑翅土白蚁工蚁成虫的触杀、忌避和传毒活性。从灰毛豆种子甲醇提取物中分离鉴定了10个化合物，即Obovatachalcone，Obovatin，异合生果素，鱼藤酮，Pongachin，12α-羟基鱼藤酮，6a,12a-脱氢灰叶酚，灰叶素，Penduletin和棉子糖。5个化合物（异合生果素，灰叶素， Obovatin，12α-羟基鱼藤酮和鱼藤酮）表现出触杀活性，处理后24 h的LD50值分别为每虫93.9，123.5，209.1，259.4和370.8μg；3个化合物（Obovatin, 鱼藤酮和12α-羟基鱼藤酮）表现出传毒活性，处理后56h累积中毒率达24.3，38.7和100%；2个化合物（异合生果素和灰叶素）在处理浓度为300 mgoL-1时表现出较弱的忌避活性。结果表明，化合物12α-羟基鱼藤酮对黑翅土白蚁工蚁成虫无忌避性，有一定的触杀活性，在群体中的毒性传播能力强，可进一步研究用于白蚁防治。

关键词：灰毛豆种子杀虫成分黑翅土白蚁杀虫活性

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Identification of compounds from the extracts of Tephrosia purpurea seeds and their insecticidal activities against Odontotermes formosanus adults

LI Guanhua ¹
李冠华，女，硕士，农艺师，生物源农药。
WANG Suning ² DING Wenbing ² XUE Zhengjie ³ LIU Zili ⁴ LI Youzhi ⁴
李有志，男，博士，副教授

1、Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128
2、Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China
3、National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China
4、Changsha station of Termite Control and Prevention, Changsha 410005, China

Abstract：In order to determine insecticidal compounds from the methanol extracts of Tephrosia purpurea seeds and their insecticidal activities against the adult of Odontotermes formosanus, the compounds were isolated with column chromatography and their structures were established by NMR spectroscopy, as well as by HR-MS analysis. The contact, repellent, transmission toxicity of each compound were determined against the adult of O. formosanus. The results were as following: Ten known compounds were isolated and identified (Obovatachalcone, Obovatin, Isolonchocarpin, Rotnone, Pongachin, 12α- hydroxyrotenone, 6α,12α-dehydrodeguelin, Tephrosin, Penduletin, Raffinose). Five compounds showed insecticidal activity against the adult of O. formosanu among the ten. The five (Isolonchocarpin, Tephrosin, Obovatin, 12α-hydroxyrotenone and Rotenone) exhibited contact toxicity with the LD50 value being 93.9, 123.5, 209.1, 259.4 and 370.8 μg per insect at 24 hours after treatment and the three (Obovatin, Rotenone and 12α-hydroxyrotenone) exhibited transmission toxicity with the cumulative poisoning rate being 24.3，38.7 and 100% at 56 hours after treatment, respectively. At the dosage of 500 mgoL-1, the two (Isolonchocarpin and Tephrosin) exhibited weak repellent toxicity. The profiles of these compounds against the adult of O. formosanu suggested that 12α-hydroxyrotenone were the most potential insecticides in O. formosanu control for the further research.

Keywords： Tephrosia purpurea seeds insecticidal compounds Odontotermes formosanus insecticidal activities

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李冠华，王苏宁，丁文兵，等. 灰毛豆种子中化学成分的鉴定及其对黑翅土白蚁的杀虫活性[EB/OL]. 北京：中国科技论文在线 [2013-03-01]. https://www.paper.edu.cn/releasepaper/content/201303-22.

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论文编号	201303-22
论文题目	灰毛豆种子中化学成分的鉴定及其对黑翅土白蚁的杀虫活性
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