Research Progress of Ionic Liquids in biological macromolecules

• 1、School of Agriculture and Bio-engineering, Tianjing University

Abstract：Ionic liquid is a new developed green solvent system for dissolving bio-macromolecule, such as protein and cellulose. Ionic liquid has intrinsically useful properties，such as good thermal stability, low melting point, negligible vapour pressure, changeable acidity, high dissolvability, low viscosity and density. The application of ionic liquid in macromolecules research has received great attention. The dissolution and crystallization behavior of protein and and the effect ionic liquids on the activity and stability of protein were summerized in this paper. The related research results indicated that ionic liquid + water system could provide hydrogen-band environment for disslution of protein, which can improve disslution behavior. The activity of enzyme is generally equivalent or higher than that of usual organic solvents and the thermal stability, operating stability and selectivity of enzyme are promoted. By adding ionic liquid to induce protein crystallization, X-ray diffraction resolution of protein could be greatly improved. Furthermore,the dissolution behavior of cellulose was also introduced in this paper.

Keywords： ionic liquids bio-macromolecule dissolution behavior crystallization