Effect of expression of β-Casein on mice\\\

• 1、Key Laboratory of Dairy Science Ministry of Education,Northeast Agricultural University

Abstract：Chinese herbs，which is made up by plants，animals and mineral，is a natural resource can be absorbed most easily by animals. And Chinese herbs ，which has less side- effect，less remains，less drug-resistance and leads less pollutions，causes better effects on dairy cows. Thus，people use Chinese herb as raw material to product the mixed additive including no antibiotics. Research and use of the additive on milk cows are the focus of current scholarly pursuit. The study，as following ，is on the level of Biochemistry and Molecular biology. We selected five Chinese herbs among the compound recipes extensively used in human beings to increase the level of milk ，they are Cowherb Seed，JiaZhu，Rhaponticum uniflorum，Medulla Tetrapanacis and Taraxacu Mogono. The herbs being boiled，we extracted the juice that includes no bacterium.The research use the cells of mammary gland of mouse that feeds its babies more than 5 days and less than 12 days. We added the herbs into the culture medium of cell respectively and did a rang of comparisons –the expression of β-Casein compared-with the circumstances without additive and only within prolactin. There are five parts in the experiment .First，we use the approach of MTT to evaluate how the herbs influence the cells on cell proliferation. Second，we studied the regulation of cyclin D1 and Stat 5 by laser scanning confocal influorescence microscopy. Third，Real time Quantitative PCR. Forth，capillary electrophoresis is used on detecting β-Casein ，too. Fifth，injecting the juice into the mammary gland of mouse on lactation to measure quantity of milk.The result indicated that the pangolin scales ，medulla tetrapanacis and rhapontici can restrain the multiplication of mouse mammary epithelial cells（P＜0.05），and cowherb seed can restrain the multiplication of mouse mammary epithelial cells extremely（P＜0.01）. But，dandelion can not restrain the multiplication of mouse mammary epithelial cells.

Keywords： MTT cyclin-D Stat 5 β-casein laser scanning confocal influorescence microscopy