Identification of two dazl splicing variants in Japanese flounder (Paralichthys olivaceus)

• 1、College of Marine Life Science, Ocean University of China

Abstract：DAZL is an RNA-binding protein of DAZ family, playing key roles in reproduction of organisms. Here in this study, we clone and identify the dazl gene in a kind of flatfish, Japanese flounder (Paralichthys olivaceus). Two different splicing variants of dazl mRNAs are found, and they differ from each other by a 51-bp insertion/deletion. We characterize the two mRNA variants by qPCR and in situ hybridization and prove their germ cell specific expression patterns. Results of western blot and immunohistochemistry show that the two isoforms of their translational production also express specifically in germ cells of both sexes. The similar expression patterns of the two variants may imply their complementary functions in germ cell development. But there are also differences in their functions. By RNA-binding protein immunoprecipitation, we find Podazl-I and Podazl-II may be the target of both PODAZL isoforms. More sequencing results will offer more detailed and accurate information on their functions. Studies on the two dazl variants in Japanese flounder are rare in teleosts. Our results may facilitate further studies on reproduction related genes and germ cell development.

keywords： Genetics dazl RNA binding protein alternative splice germ cell Japanese flounder

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