氮调控提高普通小球藻C.vulgaris C95的油脂含量的研究
首发时间:2014-03-04
摘要:实验室条件下,考察了在发酵过程中不同氮源对小球藻的生物量和油脂积累的影响;确定了小球藻的最佳氮源,并对比分析了单步培养与氮调控培养的生物量、油脂含量、氮消耗量、生物量对氮得率和相对油脂对氮得率的不同。结果表明:小球藻在16mmol/L KNO3时获得最大生物量,为562.2mg/L,在8mmol/L KNO3时获得最大相对油脂含量为11.24;培养小球藻的最佳氮源为8mmol/L KNO3;氮调控培养时,最大相对油脂含量为17.12,比单步培养高约1.4倍;单步培养时,最高生物量为626.3mg/L,比氮调控培养高约1.9倍。氮源对生物量,相对油脂含量,生物量对氮得率和相对油脂对氮得率具有明显的影响。
For information in English, please click here
Improvement of Lipid Content of Chlorella vulgaris C95 by Controled Nitrogen Cultivation
Abstract:The different nitrogen on the growth and oil accumulation of Chlorella vulgaris, and the optimum nitrogen source were studied, together with the growth, oil accumulation, nitrogen consumption, cell yield coffecient to nitrogen and lipid yield coffecient to nitrogen under the single cultivation and controled nitrogen cultivation. The results show that Nitrogen source concentration step gain maximum biomass at 16mmol/L KNO3 and maximum lipid accumlation at 8mmol/L KNO3; the optimal nitrogen source of chlorella type and concentration is 8mmol/L KNO3; Under independent cultivation; Compared with the single step training, cultivate chlorella in stages of oil content was 17.2, increased 1.4 times; the highest biomass was 626.3 mg/L under single step training, about 1.9 times higher than the controled nitrogen cultivation. These results collectively suggested that nitrogen source can obviously affect the biomass, oil content, cell yield coffecient to nitrogen and lipid yield coffecient to nitrogen. Therefore, nitrogen source could be modified to regulate physiological metabolic during the cultivation of Chlorella vulgaris.
Keywords: Fermentation Engineering Chlorella vulgaris Nitrogen source Oil accumulation Nitrogen regulation
论文图表:
引用
No.4586545959285139****
同行评议
共计0人参与
勘误表
氮调控提高普通小球藻C.vulgaris C95的油脂含量的研究
评论
全部评论0/1000